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KMID : 0861020140290060111
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Korea Journal of Herbology
2014 Volume.29 No. 6 p.111 ~ p.116
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The Antioxidant Activity of Cnidii Fructus and Torilis Fructus in Leydig cells
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Oh Ji-Hoon
Kim Do-Rim
Park Soo-Yeon
Chang Mun-Seog
Park Seong-Kyu
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Abstract
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Objectives : The purpose of this study was to estimate the antioxidant activity of water extract of Cnidii Fructus (CF) and Torilis Fructus (TF) in Leydig cells.
Methods : Free radical scavenging activity of CF and TF against 2,2-diphenyl-1-picrylhydrazyl (DPPH) was determined spectrophotometrically. We investigated the effect of CF and TF in Leydig cells by MTT assay. The protective effects of CF and TF against hydrogen peroxide-induced oxidative stress in Leydig cells. Superoxide dismutase (SOD), and catalase activity assays were performed in Leydig cells.
Results : The results showed that CF scavenged DPPH radical in a dose-dependent manner by up to 81.2%, TF scavenged DPPH radical in a dose-dependent manner by up to 63.8%. CF showed cell viability as 121.0, 132.7, 126.6% in 5, 10, 100 μg/ml concentrations. TF showed cell viability as 127.5, 111.8% in 5, 100 μg/ml concentraions, respectively. The hydrogen peroxide-induced cytotoxicity of Leydig cells were protected to 86.3% by CF at concentration of 10 μg/ml and protected to 83.5% by TF at concentration of 100 μg/ml. Both CF and TF at all concentrations, SOD activity was not significantly changed. Catalase activity was significantly increased at 10, 100 μg/ml concentrations of CF, respectively. TF`s catalase activity showed no significant difference from that of the control.
Conclusions : These results suggest that CF, as an antioxidant, protects Leydig cells in hydrogen peroxide-induced oxidative stress. know that『Kwangjebikeup』played a role in settlement and spreading of foreign knowledge to civilians.
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KEYWORD
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Cnidium monieri, L. Cussion, Torilis japonica Decandolle, Leydig cells, 2, 2-diphenyl-1-picrylhydrazyl, PPH, Cell viability, Catalase
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